Conclusion MNNG can promote the methylation of aldh1l1 gene and the expression of lrrfip1 and aldh1l1 in Kazakh normal esophageal epithelial cells### Objective to investigate the effect of adenovirus 36 infection on peroxisome proliferator activated receptor (PPAR) of human adipose derived mesenchymal stem cells γ) Methods human subcutaneous adipose tissue and type I collagenase were obtained under sterile conditions, and human adipose derived mesenchymal stem cells (hamsc) were digested and collected.
Conclusion microrna-5692b is highly expressed in Kazak esophageal squamous cell carcinoma, which can assist in the clinical diagnosis of esophageal squamous cell carcinoma### Objective to investigate the effects of N-methyl-N ‘- nitro-N-nitrosoguanidine (MNNG) on the methylation and expression of lrrfip1 and aldh1l1 genes in Kazak normal esophageal epithelial cells.
Compared with the control group, the concentration groups increased, and the difference was statistically significant (P & lt; 0.05).
Our advantages: direct connection with social leaders, stable employment, ten-year reputation brand, there is a statistically significant difference in the expression in Xinjiang Kazak esophageal squamous cell carcinoma tissues and adjacent normal tissues (P & lt; 0.05), which is highly expressed in cancer tissues, And increased with the increase of clinical stage, the difference was statistically significant (P & lt; 0.05).
Methods normal Kazakh esophageal epithelial cells cultured in vitro were exposed to 0, 0.75, 1.5 and 3 μ In the medium of g / mlmnng for 24h, it was the control group and 0.75, respectively μ G / ml group, 1.50 μ G / ml group, 3.00 μ G / ml group.
The expression of microrna-5692b was not associated with other clinicopathological features (P & gt; 0.05).
The mRNA expression levels of aldh1l1 gene in each group were (1.023± 0.254), (5.046± 0.603), (2.259± 0.030), (7.616± 1.910) respectively, which were significantly higher than those in the control group (P & lt; 0.05); The expression levels of aldh1l1 protein in cells of each group were (0.725± 0.014), (0.913± 0.033), (1.142± 0.010), (1.334± 0.047) respectively.
The cells were transferred to the third generation (P3 generation) for directional adipogenic induction and oil red O staining.
The methylation status of lrrfip1 and aldh1l1 genes was detected by methylation specific polymerase chain (MSP), and the mRNA and protein expression of lrrfip1 and aldh1l1 genes were detected by RT-PCR and Western blot.
Results compared with the control group, the methylation status of lrrfip1 gene in each concentration group did not change; The mRNA expression levels of lrrfip1 gene in each group were (1.021± 0.237), (2.828± 0.350), (1.453± 0.179), (1.952± 0.223); Compared with the control group, 0.75 μ G / ml group and 3.00 μ The expression of g / ml group increased, and the difference was statistically significant (P & lt; 0.05); Compared with the control group, 1.50 μ G / ml and 3.00 μ The expression levels of lrrfip1 protein in g / ml group increased, which were (4.233± 0.048), (4.519± 0.033), (5.377± 0.057) respectively, and the difference was statistically significant (P & lt; 0.05); In the high concentration group, aldh1l1 gene changed from partial methylation to complete methylation.
Publication of journals (provincial journals, national journals, core journals of Peking University, SCI journals, etc.), topics, patents, monographs, scientific research projects.
The surface immunophenotype of hamsc was detected by flow cytometry.
